Deuterium Oxide-induced Reversion of Naegleria gruberi Flagellates | Zendy

Terence M. Preston | Zendy; D.S. O'Dell | Zendy

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Deuterium Oxide-induced Reversion of Naegleria gruberi Flagellates

Author(s) -

Terence M. Preston,

D.S. O'Dell

Publication year - 1973

Publication title -

journal of general microbiology

Language(s) - English

Resource type - Journals

eISSN - 2059-9323

pISSN - 0022-1287

DOI - 10.1099/00221287-75-2-351

Subject(s) - reversion , naegleria , flagellate , cycloheximide , biology , naegleria fowleri , protozoa , microbiology and biotechnology , virology , biochemistry , botany , protein biosynthesis , gene , phenotype , meningoencephalitis

SUMMARY: A bead column technique is described for the separation of Naegleria gruberi flagellates from amoebae and cysts. It is shown that D2O induces a rapid reversion of these flagellates to amoebae, and the behaviour and structure of the reverting cells is described. The amoebae are viable in high concentrations of D2O, and when returned to suspension in H2O readily retransform to the flagellate stage. The reversion of flagellates to amoebae is insensitive to cycloheximide, but the subsequent retransformation to flagellate can be prevented by both actinomycin D and cycloheximide.

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