A Simple Chemically Defined Medium for the Production of Phase I Bordetella pertussis | Zendy

D. W. Stainer | Zendy; M. J. Scholte | Zendy

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A Simple Chemically Defined Medium for the Production of Phase I Bordetella pertussis

Author(s) -

D. W. Stainer,

M. J. Scholte

Publication year - 1970

Publication title -

journal of general microbiology

Language(s) - English

Resource type - Journals

eISSN - 2059-9323

pISSN - 0022-1287

DOI - 10.1099/00221287-63-2-211

Subject(s) - bordetella pertussis , bordetella , chemistry , cystine , antigen , microbiology and biotechnology , pertactin , biochemistry , biology , pertussis toxin , bacteria , immunology , enzyme , g protein , genetics , receptor , cysteine

SUMMARY A simple, chemically defined medium is described consisting of sodium glutamate, proline, cystine, salts, and growth factors, which is suitable for the large-scale production of phase I Bordetella pertussis. More than 30 x IO~ organisms/ml. were produced in 48 to 72 h. growth in shake flasks and fer- mentors. The cultures were detoxified by the addition of 0.14 % formalin to yield vaccines which were non-toxic to mice and guinea pigs and had good mouse-protective antigen levels. The antigenic stability was satisfactory after storage of the final vaccines at elevated temperatures.

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