Sedimentation Properties of Ribonucleic Acid from Rhodopseudomonas spheroides | Zendy

Leonor Sterin Borda | Zendy; M. H. Green | Zendy; M. D. Kamen | Zendy

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Sedimentation Properties of Ribonucleic Acid from Rhodopseudomonas spheroides

Author(s) -

Leonor Sterin Borda,

M. H. Green,

M. D. Kamen

Publication year - 1969

Publication title -

journal of general microbiology

Language(s) - English

Resource type - Journals

eISSN - 2059-9323

pISSN - 0022-1287

DOI - 10.1099/00221287-56-3-345

Subject(s) - rna , ribosomal rna , centrifugation , sedimentation , escherichia coli , phenol extraction , extraction (chemistry) , biochemistry , chemistry , 50s , sucrose gradient , dna , biology , microbiology and biotechnology , ribosome , chromatography , gene , enzyme , sediment , paleontology

SUMMARY: Phenol-purified RNA was prepared from Rhodopseudomonas spheroides and characterized by sucrose-gradient centrifugation. The patterns obtained indicate that R. spheroides possesses a ribosomal RNA complement similar to that of Escherichia coli. This is contrary to previous claims that the 23S component is missing. This species of ribosomal RNA was demonstrated to be more labile than the corresponding component in E. coli and variations in yields produced by changes in extraction procedures are described.

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