Open AccessSpontaneous Mutation in Spheroplasts of Escherichia colid
Author(s) -
Francis J. Ryan,
Toshihiko Okada,
Takako Nagata
Publication year - 1963
Publication title -
journal of general microbiology
Language(s) - English
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-30-2-193
Subject(s) - spheroplast , escherichia coli , mutation , biology , microbiology and biotechnology , chemistry , genetics , gene
SUMMARY: Spheroplast suspensions containing as few as one rod in a thousand were made with Escherichia coli (15 his-) by the use of penicillin. These spheroplasts could be reverted to the rod state with efficiency approaching 100%. Spontaneous mutation from histidine requirement to non-requirement was observed in these spheroplasts, in which cell division did not take place.
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