Open AccessIdentification of staphylococcal and streptococcal causes of bovine mastitis using 16S-23S rRNA spacer regions
Author(s) -
Pivi Forsman,
Anu Tilsaia-Timisjrvi,
Tapani Alatossava
Publication year - 1997
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/00221287-143-11-3491
Subject(s) - streptococcus dysgalactiae , streptococcus uberis , mastitis , biology , streptococcus agalactiae , microbiology and biotechnology , staphylococcus , staphylococcus epidermidis , staphylococcus aureus , streptococcus , staphylococcus intermedius , ribosomal rna , genetics , bacteria , gene
Bovine mastitis is caused mainly by certain Staphylococcus and Streptococcus species. The sequences of the 16S-23S rRNA spacer regions were determined for the nine species which cause mastitis: Staphylococcus aureus, Staphylococcus chromogenes, Staphylococcus epidermidis. Staphylococcus hyicus, Staphylococcus simulans, Staphylococcus xylosus. Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis. Significant variation was found between the spacer sequences of different species with the lengths of the spacers varying from 240 to 461 bp. Between genera the spacers shared only short conserved regions (8-9 bp) and within genera the sequence identities varied from 53 to 85%. This variation made it possible to construct specific primer pairs for these species and genera. The specificities of these primers were tested with 25 bacterial species and 51 isolates from cattle with clinical mastitis. The DNA-based identification of the mastitis species was mostly successful.