Open AccessIdentification and characterization of phosphoenolpyruvate: fructose phosphotransferase systems in three Streptomyces species
Author(s) -
Fritz Titgemeyer,
Jürgen Walkenhorst,
Jonathan Reizer,
Maarten H. Stuiver,
Xuewen Cui,
Milton H. Saier
Publication year - 1995
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/00221287-141-1-51
Subject(s) - phosphoenolpyruvate carboxykinase , pep group translocation , streptomyces coelicolor , biochemistry , fructose , enzyme , phosphotransferase , biology , streptomyces , phosphatase , microbiology and biotechnology , fructose bisphosphate aldolase , bacteria , pyruvate kinase , chemistry , aldolase a , glycolysis , genetics
Streptomyces lividans, S. coelicolor and S. griseofuscus were examined for the presence of the enzymes of the phosphoenolpyruvate:sugar phosphotransferase system (PTS). All three species were shown to possess Enzyme I, HPr and fructose-specific Enzyme II (IIFru) activities. In S. lividans and S. coelicolor, all three PTS enzymes were fructose-inducible, but in S. griseofuscus the system was expressed constitutively. These organisms apparently lack the HPr(Ser) kinase and HPr(Ser-P) phosphatase that characterize low-GC Gram-positive bacteria.