Open AccessComparison of PCR fingerprinting, by random amplification of polymorphic DNA, with other molecular typing methods for Candida albicans
Author(s) -
A Bostock,
Muhammad Naeem Khattak,
Ruth Matthews,
James Burnie
Publication year - 1993
Publication title -
journal of general microbiology/journal of general microbiology
Language(s) - English
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-139-9-2179
Subject(s) - rapd , pulsed field gel electrophoresis , typing , biology , ecori , primer (cosmetics) , dna profiling , restriction fragment length polymorphism , genetics , candida albicans , dna , polymerase chain reaction , microbiology and biotechnology , restriction enzyme , genotype , gene , chemistry , genetic diversity , medicine , population , environmental health , organic chemistry
Fingerprinting by random amplification of polymorphic DNA (RAPD) was compared with existing molecular typing systems for Candida albicans. Fifteen isolates were chosen, including three from the same patient; these gave 14 distinct karyotypes by pulsed-field gel electrophoresis (PFGE) and 7 different DNA types by EcoRI-generated restriction fragment length polymorphisms (RFLPs). RAPD with primer I (5'GCTGGTGG3') gave 5 types, whereas primer II (5'GCGCACGG3') yielded 11 types. Combining the results from both primers, all isolates were unique by RAPD with the exception of the three from the same patient. RAPD provided a fast, economical and reproducible means of typing C. albicans with a level of discrimination approaching that of PFGE.