Open AccessCharacterization of bacteriophage BFK20 from Brevibacterium flavum
Author(s) -
Michael Koptides,
Imrich Barák,
Marta Šišová,
Edita Baloghová,
Jana Ugorčaková,
Jozef Timko
Publication year - 1992
Publication title -
journal of general microbiology/journal of general microbiology
Language(s) - English
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-138-7-1387
Subject(s) - brevibacterium , bacteriophage , capsid , dna , biology , genome , microbiology and biotechnology , strain (injury) , chemistry , escherichia coli , gene , bacteria , genetics , microorganism , anatomy
Bacteriophage BFK20 was isolated from a Brevibacterium flavum strain that had become contaminated during industrial fermentation. BFK20 has a polyhedral head 50 nm wide and a non-contractile tail 200 nm long and 10 nm in diameter. The genome of this bacteriophage consists of a linear double stranded DNA molecule of 44-45 kb with cohesive ends. The capsid of phage BFK20 contains nine polypeptides with molecular masses from 22.0-108.0 kDa. BFK20 DNA was used as a donor for fragments carrying promoters and transcription-terminators.