Open AccessHyperpolarization and intracellular acidification in Trichoderma viride as a response to illumination
Author(s) -
Miroslav Grešík,
N. Kolarova,
Vladimı́r Farkaš
Publication year - 1991
Publication title -
journal of general microbiology/journal of general microbiology
Language(s) - English
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-137-11-2605
Subject(s) - hyperpolarization (physics) , antimycin a , valinomycin , intracellular , membrane potential , ionophore , biophysics , chemistry , efflux , intracellular ph , cytoplasm , biochemistry , cytoplasmic streaming , biology , mitochondrion , membrane , stereochemistry , nuclear magnetic resonance spectroscopy
Using indirect methods based on uptake of [3H]tetraphenylphosphonium cation and [14C]benzoic acid by cells of the fungus Trichoderma viride we found that the illumination-induced transient hyperpolarization of the plasma membrane is followed immediately by a rapid temporary decrease in intracellular pH. Hyperpolarization and intracellular acidification were completely suppressed by 150 mM-KCl and by the K(+)-ionophore valinomycin. The light-induced acidification of the cytoplasm was not observed in the presence of the cytochrome respiratory chain inhibitors antimycin A and mucidin. Based on these results, we hypothesize that the hyperpolarization of the cells is the consequence of an efflux of K+ through a light-activated K(+)-channel in the plasma membrane. The loss of positive charge in the cytoplasm caused by this efflux of cations is counterbalanced by H+ originating from the light-activated mitochondrial respiratory chain.