Open AccessLipids of Candida albicans: subcellular distribution and biosynthesis
Author(s) -
Nandini Mago,
G. K. Khuller
Publication year - 1990
Publication title -
journal of general microbiology/journal of general microbiology
Language(s) - English
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-136-6-993
Subject(s) - biosynthesis , phosphatidylcholine , ethanolamine , phosphatidylserine , phosphatidylinositol , biochemistry , candida albicans , chemistry , choline , serine , phosphatidylethanolamine , pi , phospholipid , chromatography , biology , enzyme , microbiology and biotechnology , membrane , phosphorylation
Lipids constituted around 5% of the dry weight in Candida albicans 3153, while sterols and phospholipids accounted for 1.2% and 1.1% respectively. Phospholipids were mainly localized in the microsomal fraction; phosphatidylserine (PS), phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) were the major phospholipids. Incorporation studies with [14C]acetate and [32P]orthophosphoric acid demonstrated that PS was synthesized at the highest rate followed by PC, PE and PI. There was little difference in either the content of the rate of biosynthesis of PC, PE and PI. Incorporation of labelled serine, ethanolamine and choline revealed serine to be a precursor for PC, PE and PS, ethanolamine for PC and PE, and choline for PC biosynthesis only.