Open AccessConjugative plasmid transfer from Escherichia coli to Clostridium acetobutylicum
Author(s) -
Denise Williams,
Danielle I. Young,
Michael Young
Publication year - 1990
Publication title -
journal of general microbiology/journal of general microbiology
Language(s) - English
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-136-5-819
Subject(s) - clostridium acetobutylicum , plasmid , shuttle vector , escherichia coli , clostridium butyricum , microbiology and biotechnology , replicon , electroporation , biology , enterococcus faecalis , clostridium , escherichia , bacteria , genetics , gene , recombinant dna , vector (molecular biology) , biochemistry , butanol , ethanol
The conjugation mechanism of IncP plasmids may be employed to mobilize small non-conjugative plasmids from Escherichia coli to a wide range of different organisms. This strategy has been adapted for use with the Gram-positive anaerobe, Clostridium acetobutylicum NCIB 8052. Several shuttle vectors containing replicons from pAM beta 1 (Enterococcus faecalis), pCB101 (Clostridium butyricum) or pWV01 (Streptococcus cremoris), together with the cis-acting oriT region of RK2, have been constructed, and transferred to and established in this organism. One of the vectors apparently contains a hot spot for insertion of IS1. Conjugative mobilization of plasmids from E. coli will provide a useful alternative to electroporation for effecting gene transfer to this industrially important anaerobe.