Open AccessMolecular Analysis of -Lactamases from Four Species of Streptomyces: Comparison of Amino Acid Sequences with those of other -clactamases
Author(s) -
Mats Forsman,
Britta Häggström,
Lena Lindgren,
Bengtåke Jaurin
Publication year - 1990
Publication title -
journal of general microbiology/journal of general microbiology
Language(s) - English
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-136-3-589
Subject(s) - streptomyces , streptomyces fradiae , biology , gene , streptomycetaceae , bacteria , homology (biology) , genetics , sequence analysis , actinomycetales
Genes encoding extracellular beta-lactamases of Streptomyces badius, Streptomyces cacaoi, Streptomyces fradiae and Streptomyces lavendulae were cloned and mapped in Streptomyces lividans. DNA sequence analysis of the beta-lactamase genes revealed a high overall G + C content, ranging from 71 to 75 mol%, with a G + C content of 95 mol% at the third position of the codons for all four genes. The primary structure of the beta-lactamases including their signal peptides was deduced. The four beta-lactamases exhibited homology to each other and to class A beta-lactamases from other bacterial genera. We suggest that Streptomyces beta-lactamases are representatives of a superfamily of genes, from which class A beta-lactamases of Gram-negative bacteria may have evolved.