Open AccessIron Regulated Outer Membrane Proteins of Escherichia coli: Variations in Expression Due to the Chelator Used to Restrict the Availability of Iron
Author(s) -
Henrik Chart,
Martin Buck,
P. Stevenson,
Elwyn Griffiths
Publication year - 1986
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/00221287-132-5-1373
Subject(s) - escherichia coli , enterobactin , bacterial outer membrane , ovotransferrin , chelation , chemistry , ferric , bacteria , siderophore , enterobacteriaceae , aerobactin , biochemistry , ferrous , microbiology and biotechnology , ferric iron , transferrin , biology , inorganic chemistry , gene , genetics , organic chemistry
Iron restriction was induced in Escherichia coli O 111, E. coli O 164 and E. coli C by growing the organisms in trypticase soy broth containing ovotransferrin, desferal, EDDA (ethylenediamine-dihydroxyphenylacetic acid) or alpha,alpha'-dipyridyl. There were marked qualitative and quantitative differences in the iron regulated outer membrane proteins expressed in the presence of the various iron chelators. Differences in the kinetics of growth were also noted. E. coli C was devoid of a ferric enterobactin iron uptake system.