Characterization of a Conjugative Plasmid From Erwinia stewartii | Zendy

D. L. Coplin | Zendy; Reid D. Frederick | Zendy; Sally L. McCammon | Zendy

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Characterization of a Conjugative Plasmid From Erwinia stewartii

Author(s) -

D. L. Coplin,

Reid D. Frederick,

Sally L. McCammon

Publication year - 1985

Publication title -

microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.352

H-Index - 35

eISSN - 1465-2080

pISSN - 1350-0872

DOI - 10.1099/00221287-131-11-2985

Subject(s) - plasmid , escherichia coli , biology , enterobacteriaceae , microbiology and biotechnology , shigella flexneri , transposable element , strain (injury) , genetics , gene , mutant , anatomy

Summary: pDC250 is a 52 kb conjugative cryptic plasmid from Erwinia stewartii strain SW2. This plasmid and its transposon-carrying derivatives, pDC251 and pDC252, were transmissible to E. chrysanthemi, E. amylovora, E. milletiae, E. herbicola, Escherichia coli, Shigella flexneri and Salmonella arizonae. In Esch. coli, E. stewartii and E. chrysanthemi, pDC251 was stable and derepressed for conjugal transfer. Hybridization studies confirmed that pDC250 is widely distributed among E. stewartii strains and showed that it was also closely related to 34 kb plasmids found in strains lacking a 52 kb plasmid. The 34 kb plasmid from E. stewartii strain SS104, pDC140, was transformed into Esch. coli and compared with pDC250. Restriction mapping of the two plasmids revealed a nearly identical 20 kb region. In Esch. coli, pDC140 was unstable in the presence of pDC250.

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