Cloning of the Bacillus subtilis spoIIA and spoVA Loci in Phage 105DI:1t | Zendy

D. Savva | Zendy; J. Mandelstam | Zendy

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Cloning of the Bacillus subtilis spoIIA and spoVA Loci in Phage 105DI:1t

Author(s) -

D. Savva,

J. Mandelstam

Publication year - 1984

Publication title -

microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 179

eISSN - 1465-2080

pISSN - 1350-0872

DOI - 10.1099/00221287-130-8-2137

Subject(s) - bacillus subtilis , recombinant dna , dna , biology , cloning (programming) , microbiology and biotechnology , hindiii , molecular cloning , genetics , bacteriophage , genomic library , plasmid , escherichia coli , gene , bacteria , base sequence , peptide sequence , computer science , programming language

A 6.95 kb HindIII-generated DNA fragment from Bacillus subtilis 168 was inserted into the DNA of phage phi 105DI:1t. The recombinant phage (phi 105DS1) contained DNA of 33.8 kb as compared with 35.2 kb for phi 105DI:1t and 39.2 kb for the wild-type phage. In the presence of helper phage, phi 105DS1 complemented both spoIIA and spoV A mutations in B. subtilis.

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