Open AccessIsolation and Mapping of Glutathione Reductase-negative Mutants of Escherichia coli K12
Author(s) -
Nathan Davis,
Sheldon Greer,
M. C. JonesMortimer,
Richard N. Perham
Publication year - 1982
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/00221287-128-7-1631
Subject(s) - prophage , escherichia coli , glutathione reductase , mutant , bacteriophage , glutathione , strain (injury) , lysogenic cycle , biology , microbiology and biotechnology , mutation , gene , chemistry , genetics , biochemistry , enzyme , glutathione peroxidase , anatomy
Two independent mutants defective in glutathione reductase (EC 1.6.4.2) were isolated in an Escherichia coli K12 strain lysogenized with bacteriophage Mu. The prophage was lost (and the ability to reduce glutathione regained) by 32% of the xylose-positive transductants when T4GT7 was used as the vector, but the markers were not cotransduced by P1. Similarly, the prophage site and malA were cotransduced by T4GT7 but not by P1. The gor gene maps between min 77 and 78 on the E. coli genome, and the mutation causes no growth defect.