Open AccessCysteine Auxotrophs of Salmonella typhimurium which Grow without Cysteine in a Hydrogen/Carbon Dioxide Atmosphere
Author(s) -
Ericka L. Barrett,
GuGang Chang
Publication year - 1979
Publication title -
journal of general microbiology/journal of general microbiology
Language(s) - English
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-115-2-513
Subject(s) - cysteine , auxotrophy , chemistry , mutant , sulfur , salmonella , carbon dioxide , hydrogen , incubation , wild type , microbiology and biotechnology , biochemistry , biology , bacteria , enzyme , gene , genetics , organic chemistry
Cysteine auxotrophs of Salmonella typhimurium mutated in cysB, cysI or cysJ grew with sulphate as a sulphur source when incubated under a hydrogen/carbon dioxide atmosphere. Yields obtained under these conditions were equivalent to those characteristic of wild-type S. typhimurium. The same mutants failed to grow with sulphate as a sulphur source when incubated aerobically. Auxotrophs mutated in cysA, cysC, cysD, cysE, cysG and cysH required cysteine for growth under both incubation conditions. The results suggest that mutations in cysB (regulation of the several cys operons) and also cysI and cysJ (sulphite reductase activity) can be circumvented during anaerobic growth under hydrogen.