Open AccessEffect of R Plasmid RP1 on the Nutritional Requirements of Escherichia coli in Batch Culture
Author(s) -
Ruth M. M. Klemperer,
Nahla T. A. J. Ismail,
M. R. W. Brown
Publication year - 1979
Publication title -
journal of general microbiology/journal of general microbiology
Language(s) - English
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-115-2-325
Subject(s) - escherichia coli , plasmid , phosphate , nutrient , growth rate , enterobacteriaceae , biology , chemistry , microbiology and biotechnology , biochemistry , dna , gene , ecology , mathematics , geometry
The minimal nutritional requirements of Escherichia coli have been quantitatively determined in batch culture for cells with (R+) and without (R-) the R plasmid RP1. In these conditions R+ cells have a greater requirement than R- for several nutrients, particularly Mg2+, K+, Fe2+ and PO43-. The maximum growth rate in a simple salts medium was the same for R+ and R- cells. At low concentrations of phosphate, the specific growth rate of R+ cells differed from that found for R- cells. The R plasmid was stable in simple salts medium, irrespective of the nutrient ultimately depleted by growth, but, on storage, R+ cells survived for a shorter time than R- cells.