DNA-mediated Genetic Changes in Neurospora crassa | Zendy

N.C. Mishra | Zendy

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DNA-mediated Genetic Changes in Neurospora crassa

Author(s) -

N.C. Mishra

Publication year - 1979

Publication title -

journal of general microbiology

Language(s) - English

Resource type - Journals

eISSN - 2059-9323

pISSN - 0022-1287

DOI - 10.1099/00221287-113-2-255

Subject(s) - neurospora crassa , crassa , transformation (genetics) , dna , nuclease , genetics , biology , gene , neurospora , mutant

Evidence for genetic transformation in Neurospora crassa is based on the observations that allo-DNA has a specific effect in producing transformants which is abolished by DNAase treatment and that iso-DNA is not effective in transformation. Here, unambiguous evidence for genetic transformation is provided by transfer of a temperature-sensitive inositol requirement from a donor to a recipient strain. Data provided also suggest the role of growth conditions and the involvement of a nuclease gene in the DNA uptake and transformation of N. crassa.

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