Open AccessDenaturation Map of the ColE1-Km Plasmid pCR11
Author(s) -
Lesley W. Coggins
Publication year - 1979
Publication title -
journal of general microbiology/journal of general microbiology
Language(s) - English
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-113-1-189
Subject(s) - cole1 , plasmid , microbiology and biotechnology , cytosine , biology , ecori , thymine , genetics , kanamycin , guanine , dna , gene , nucleotide
The denaturation map of EcoRI-digested pCR11, a ColE1-Km plasmid, is described. The 2.0 kilobase ColE1-derived segment contains an adenine+thymine rich site in the colicin immunity gene region. In the 7.2 kilobase kanamycin resistance region, the transposon Tn903 consists of an adenine+thymine rich 0.98 kilobase kan gene region flanked by a guanine+cytosine rich 1.09 kilobase inverted duplication.