Identification of a New Membrane-associated Polypeptide Specified by the Coronavirus Infectious Bronchitis Virus | Zendy

A. R. Smith | Zendy; M. E. G. Boursnell | Zendy; M. M. Binns | Zendy; T. D. K. Brown | Zendy; Stephen Inglis | Zendy

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Identification of a New Membrane-associated Polypeptide Specified by the Coronavirus Infectious Bronchitis Virus

Author(s) -

A. R. Smith,

M. E. G. Boursnell,

M. M. Binns,

T. D. K. Brown,

Stephen Inglis

Publication year - 1990

Publication title -

journal of general virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.55

H-Index - 167

eISSN - 1465-2099

pISSN - 0022-1317

DOI - 10.1099/0022-1317-71-1-3

Subject(s) - virology , coronavirus , biology , infectious bronchitis virus , avian infectious bronchitis virus , coronaviridae , virus , bronchitis , identification (biology) , microbiology and biotechnology , covid-19 , infectious disease (medical specialty) , medicine , pathology , ecology , botany , disease

Nucleotide sequences from the third open reading frame of mRNA D (D3) of infectious bronchitis virus (IBV) were expressed in bacteria as part of a fusion protein with beta-galactosidase. Antiserum raised in rabbits against this fusion protein immunoprecipitated from IBV-infected chick kidney or Vero cells a polypeptide of 12.4K, the size expected for a D3-encoded product. The D3 polypeptide is apparently non-glycosylated, and appears to be associated with the membrane fraction of infected cells, as judged by cell fractionation and immunofluorescence.

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