The Complete Nucleotide Sequence of Potato Virus X and Its Homologies at the Amino Acid Level with Various Plus-stranded RNA Viruses | Zendy

Marianne J. Huisman | Zendy; Huub J. M. Linthorst | Zendy; John F. Bol | Zendy; B.J.C. Cornelissen | Zendy

Open Access

The Complete Nucleotide Sequence of Potato Virus X and Its Homologies at the Amino Acid Level with Various Plus-stranded RNA Viruses

Author(s) -

Marianne J. Huisman,

Huub J. M. Linthorst,

John F. Bol,

B.J.C. Cornelissen

Publication year - 1988

Publication title -

journal of general virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.55

H-Index - 167

eISSN - 1465-2099

pISSN - 0022-1317

DOI - 10.1099/0022-1317-69-8-1789

Subject(s) - biology , potato virus x , orfs , virology , nucleic acid sequence , rna , virus , potexvirus , complementary dna , open reading frame , plant virus , cucumber mosaic virus , microbiology and biotechnology , peptide sequence , genetics , dna , gene , coat protein

Double-stranded cDNA of potato virus X (PVX) genomic RNA has been cloned and sequenced. The sequence [6435 nucleotides excluding the poly(A) tract] revealed five open reading frames (ORFs) which were numbered one to five starting at the 5' terminus of the RNA. They encoded proteins of Mr 165588 (166K), 24622 (25K), 12324 (12K), 7595 (8K) and 25080 (coat protein), respectively. ORFs 1 and 2 were inphase coding regions. The ORF 1 product contained domains of homology with the tobacco mosaic virus 126K and 183K products. The ORF 2 and 3 products showed homologies with the barley stripe mosaic virus 58K and 14K proteins, the beet necrotic yellow vein virus 42K and 13K products and the white clover mosaic virus 26K and 13K products, respectively. The significance of these homologies with respect to putative functions of the PVX-encoded proteins are discussed.

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