Open AccessDevelopment of a combined filtration-enrichment culture followed by a one-step duplex PCR technique for the rapid detection of Campylobacter jejuni and C. coli in human faecal samples
Author(s) -
Naoaki Misawa,
Kumiko Kawashima,
Hidekazu Kawamoto,
Fusao Kondo
Publication year - 2002
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/0022-1317-51-1-86
Subject(s) - campylobacter jejuni , campylobacter , bacteria , biology , feces , microbiology and biotechnology , enrichment culture , filtration (mathematics) , campylobacter coli , polymerase chain reaction , enterobacteriaceae , chromatography , escherichia coli , chemistry , gene , biochemistry , statistics , genetics , mathematics
A new combined filtration-enrichment culture followed by a PCR technique for the rapid detection of Campylobacterjejuni and C. coli in human faeces has been developed. Only bacteria that passed through the membrane could multiply in the enrichment culture; target bacteria were detected by a one-step duplex PCR technique with combinations of primers that are specific for different Campylobacter spp., which should allow for the detection of a mixed infection in a single patient. A Falcon cell-culture insert and 24-well tissue-culture plates were used. After 2 days, both C. jejuni and C. coli were reliably detected in diluted faeces that were seeded with as few as 10 cells which corresponds to a concentration of 10(3) cfu/g. Even allowing for the dilution of faecal samples, this represents an increase in sensitivity of two-to-three orders of magnitude over the conventional filtration method.