Open AccessClonal origin of aminoglycoside-resistant Citrobacter freundii isolates in a Danish county
Author(s) -
Niels NørskovLauritsen,
Dorthe Sandvang,
Jakob Hedegaard,
Vivian Fussing,
Karen Vibeke Mortensen,
Hans Uffe SperlingPetersen,
Henrik Carl Schønheyder
Publication year - 2001
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/0022-1317-50-7-636
Subject(s) - integron , citrobacter freundii , microbiology and biotechnology , biology , aminoglycoside , plasmid , multilocus sequence typing , typing , strain (injury) , virology , gene , antibiotic resistance , genetics , enterobacteriaceae , escherichia coli , antibiotics , genotype , anatomy
During 1997, attention was drawn to an increased frequency of aminoglycoside-resistant Citrobacterfreundii in a Danish county, when a total of 24 resistant C. freundii isolates was detected. In this study, 15 such isolates were typed by pulsed-field gel electrophoresis, riboprinting and partial sequencing of the gene encoding translation initiation factor 2. Fourteen of the 15 isolates were identical, as evaluated by their antibiograms and by all these typing methods. This epidemic strain harboured the aminoglycoside resistance genes aac(3)-II and ant(3")-I, with the latter located in tandem with a dihydrofolate reductase gene in a class I integron. The source of the strain remains unresolved. Representative isolates were obtained from various specimens from hospitals and general practice throughout the county, with no evidence of patient-to-patient transmission.