Open AccessEnhanced production of vascular endothelial growth factor by human monocytic cells stimulated with endotoxin through transcription factor SP-1
Author(s) -
Tetsuya Sakuta,
Kazunobu Matsushita,
Noboru Yamaguchi,
Tohru Oyama,
Rie Motani,
Toshihiko Kinoshita,
Shigetaka Nagaoka,
Kazuhiro Abeyama,
Ikuro Maruyama,
Haruhiko Takada,
Mitsuo Torii
Publication year - 2001
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/0022-1317-50-3-233
Subject(s) - vascular endothelial growth factor , lipopolysaccharide , microbiology and biotechnology , escherichia coli , transcription factor , messenger rna , gene expression , biology , transcription (linguistics) , vascular endothelial growth factor a , chemistry , vegf receptors , gene , cancer research , immunology , biochemistry , linguistics , philosophy
The effect of endotoxin on the regulation of vascular endothelial growth factor (VEGF) mRNA expression in human monocytic (THP-1) cells was examined. Endotoxic lipopolysaccharide (LPS) from Escherichia coli and synthetic E. coli-type lipid A (LA-15-PP) enhanced VEGF mRNA expression. LPS-induced VEGF mRNA accumulation was regulated, at least in part, at the transcriptional level. Enhancement of VEGF gene expression by LPS was shown by gel shift analysis and use of transcription factor inhibitors to be mediated via the activation of SP-1.