Open AccessIsolation and characterisation of a novel spirochaete from severe virulent ovine foot rot
Author(s) -
İbrahim Demirkan,
S.D. Carter,
Craig Winstanley,
KD Bruce,
Neil Mcnair,
Marian R. Woodside,
C. A. Hart
Publication year - 2001
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/0022-1317-50-12-1061
Subject(s) - spirochaete , virulence , microbiology and biotechnology , biology , foot rot , polyclonal antibodies , isolation (microbiology) , antiserum , virology , bacteria , immunology , antibody , botany , genetics , gene , biochemistry
A novel spirochaete was isolated from a case of severe virulent ovine foot rot (SVOFR) by immunomagnetic separation with beads coated with polyclonal anti-treponemal antisera and prolonged anaerobic broth culture. The as yet unnamed treponeme differs considerably from the only other spirochaete isolated from ovine foot rot as regards morphology, enzymic profile and 16S rDNA sequence. On the basis of 16S rDNA, it was most closely related to another unnamed spirochaete isolated from cases of bovine digital dermatitis in the USA, raising the possibility of cross-species transmission. Further information is required to establish this novel ovine spirochaete as the cause of SVOFR.