Open AccessChloride secretion induced by thermostable direct haemolysin of Vibrio parahaemolyticus depends on colonic cell maturation
Author(s) -
Akira Takahashi,
Tetsuya Iida,
Rochman Naim,
Yutaka Naykaya,
Takeshi Honda
Publication year - 2001
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/0022-1317-50-10-870
Subject(s) - vibrio parahaemolyticus , secretion , hemolysin , microbiology and biotechnology , vibrionaceae , biology , toxin , microbial toxins , bacteria , chemistry , virulence , biochemistry , gene , genetics
Vibrio parahaemolyticus produces a thermostable direct haemolysin (TDH) that has been implicated in the pathogenesis of diarrhoeal disease caused by this organism. In previous work, TDH induced Cl- secretion in human colonic epithelial cells that was dependent on the intracellular Ca2+ concentration, [Ca2+]in. This study investigated whether Cl- secretion induced by TDH is influenced by the stage of maturation of intestinal epithelial cells. Two different human colonic cell lines, villus cell-like Caco-2 cells and crypt cell-like T84 cells, cultured by different methods to obtain differentiated samples, were used. When these cells were exposed to butyrate, a transcriptional regulator of differentiation genes, or co-cultured with 18Co cells, a human colonic fibroblast cell line, they showed increased trans-epithelial resistance and villus cell marker enzyme activity. In Caco-2 cells, exposure to butyrate or co-culturing with 18Co cells resulted in increased TDH binding, higher short-circuit currents (Isc) and greater [Ca2+]in. These results suggest that sensitivity to TDH is affected by the stage of cellular differentiation of cultured intestinal epithelial cells.