Open AccessModulation of actA gene expression in Listeria monocytogenes by iron
Author(s) -
Maria Pia Conte,
Catia Longhi,
G Petrone,
Mario Polidoro,
Piera Valenti,
L Seganti
Publication year - 2000
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/0022-1317-49-8-681
Subject(s) - listeria monocytogenes , enterocyte , intracellular , microbiology and biotechnology , gene expression , caco 2 , gene , biology , listeria , transcription (linguistics) , endogeny , bacteria , chemistry , cell , biochemistry , genetics , small intestine , linguistics , philosophy
This study analysed the invasiveness of Listeria monocytogenes into enterocyte-like Caco-2 cells in which iron depletion was achieved by picolinic acid treatment. Both entry and intracellular multiplication varied depending on the endogenous iron content of bacterial and eukaryotic cells. The behaviour within enterocytes was correlated with a 10-fold increased transcription of the actA gene observed in bacterial cells grown under conditions of iron stress.