The Presence of C-type RNA Virus Particles in a Rat Embryo Cell Line Spontaneously Transformed in Tissue Culture | Zendy

Louis Gazzolo | Zendy; D Šimkovič | Zendy; M. Cl. MartinBerthelon | Zendy

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The Presence of C-type RNA Virus Particles in a Rat Embryo Cell Line Spontaneously Transformed in Tissue Culture

Author(s) -

Louis Gazzolo,

D Šimkovič,

M. Cl. MartinBerthelon

Publication year - 1971

Publication title -

journal of general virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.55

H-Index - 167

eISSN - 1465-2099

pISSN - 0022-1317

DOI - 10.1099/0022-1317-12-3-303

Subject(s) - nucleoid , rous sarcoma virus , virus , biology , virology , rna , viral transformation , budding , embryo , in vitro , cell culture , microbiology and biotechnology , gene , genetics , escherichia coli

SummaryTwo established rat cell lines, WERC and TWERC, were studied for the presence of C-type RNA virus particles. WERC cells, spontaneously transformed in vitro, continuously released C-type RNA virus (WERC virus) by budding from the plasma membrane. Some of these virus particles (diameter about 100 nm.) showed an electron-lucent nucleoid of diameter 75 to 80 nm. and represented immature C-type virus. Others showed dense nucleoids of about 65 to 70 nm. diameter and looked like mature forms of murine leukaemia virus. Each cell showed 60 to 100 virus particles. The buoyant density of the virus particles in sucrose gradients was that expected for avian or murine RNA virus particles (1.16 g./ml.).No virus particles were observed in the subline of cells (TWERC) originating from the malignant transformation with avian sarcoma virus (the prague strain of Rous sarcoma virus) of parental WERC cells.

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