Open AccessReclassification of a Polycyclic Aromatic Hydrocarbon-Metabolizing Bacterium, Beijerinckia sp. Strain B1, as Sphingomonas yanoikuyae by Fatty Acid Analysis, Protein Pattern Analysis, DNA-DNA Hybridization, and 16S Ribosomal DNA Sequencing
Author(s) -
Ashraf A. Khan,
Rongfu Wang,
WeiWen Cao,
Wirt Franklin,
Carl E. Cerniglia
Publication year - 1996
Publication title -
international journal of systematic bacteriology
Language(s) - English
Resource type - Journals
eISSN - 1070-6259
pISSN - 0020-7713
DOI - 10.1099/00207713-46-2-466
Subject(s) - biology , biochemistry , gel electrophoresis , dna , microbiology and biotechnology , temperature gradient gel electrophoresis , sphingomonas , gene , 16s ribosomal rna
A bacterium isolated from a polluted stream, capable of metabolizing biphenyl, naphthalene, phenanthrene, and higher-molecular-weight polycyclic aromatic hydrocarbons (D. Gibson, V. Mahadevan, D. Jerina, H. Yagi, and H. Yeh, Science 189:295-297, 1975), was previously identified as Beijerinckia sp. strain B1. In this investigation, 16S rRNA gene sequencing, biochemical tests, fatty acid methyl ester analysis, polyacrylamide gel electrophoresis of protein, and DNA-DNA hybridization were used to determine the taxonomic relationship of Beijerinckia sp. strain B1. The sequence of the 16S rRNA gene of B1 was identical to that of Sphingomonas yanoikuyae ATCC 51230T. The biochemical tests, fatty acid analysis, and sodium dodecyl sulfate-polacrylamide gel electrophoresis profile of soluble proteins of strain B1 showed results similar to those of S. yanoikuyae. DNA-DNA hybridization indicated that B1 and S. yanoikuyae ATCC 51230T are 75% homologous at the DNA level. We propose that Beijerinckia sp. strain B1 be reclassified as S. yanoikuyae.