Open AccessPseudomonas flavescens sp. nov., Isolated from Walnut Blight Cankers
Author(s) -
D. C. Hildebrand,
Norberto J. Palleroni,
M. Hendson,
John E. Toth,
J. L. Johnson
Publication year - 1994
Publication title -
international journal of systematic bacteriology
Language(s) - Uncategorized
Resource type - Journals
eISSN - 1070-6259
pISSN - 0020-7713
DOI - 10.1099/00207713-44-3-410
Subject(s) - 16s ribosomal rna , pseudomonas , biology , microbiology and biotechnology , genomic dna , strain (injury) , ribosomal rna , pigment , pseudomonadaceae , bacteria , gene , dna , pseudomonadales , oxidase test , genetics , biochemistry , chemistry , enzyme , organic chemistry , anatomy
Two pseudomonad strains that produce a yellow cellular pigment, in addition to a diffusible fluorescent pigment on Kings medium B, were isolated from cankers on walnut trees. Biochemical properties, such as a positive oxidase reaction, a negative arginine dihydrolase reaction, and the production of a fluorescent pigment, in addition to the results of an extensive nutritional characterization study and DNA-DNA hybridization experiments, indicated that these strains belong to a new Pseudomonas rRNA group I species. This conclusion was supported by the results of a determination of the sequence of the PCR-amplified 16S rRNA gene and a comparison with the 16S rRNA genes of other bacterial species. The genomic DNAs of the strains had a base composition of 63 mol% G+C. The name Pseudomonas flavescens sp. nov. is proposed. Strain B62 (= NCPPB 3063) is the type strain of the species.