Open AccessNotes: Identity of V Factor in Culture Medium Used for Prior Growth of Two Strains of Staphylococcus aureus
Author(s) -
Donald F. Niven,
Linda Le Blanc
Publication year - 1992
Publication title -
international journal of systematic bacteriology
Language(s) - English
Resource type - Journals
eISSN - 1070-6259
pISSN - 0020-7713
DOI - 10.1099/00207713-42-4-642
Subject(s) - staphylococcus aureus , nicotinamide , nad+ kinase , stationary phase , microbiology and biotechnology , chemistry , nucleotide , identity (music) , biology , biochemistry , stereochemistry , chromatography , bacteria , genetics , physics , gene , enzyme , acoustics
Staphylococcus aureus ATCC 12598 and ATCC 25923 were starved of pyridine nucleotides and precursors and then grown in a semidefined medium containing [carbonyl-14C]nicotinamide. Samples of medium from late-exponential-phase and stationary-phase cultures were analyzed for 14C-metabolites. In all cases, V factor was present primarily as NAD.