Open AccessPhylogenetic Relationships of Three Porcine Mycoplasmas, Mycoplasma hyopneumoniae, Mycoplasma flocculare, and Mycoplasma hyorhinis, and Complete 16S rRNA Sequence of M. flocculare
Author(s) -
Gerald W. Stemke,
Frédéric Laigret,
Odile Grau,
Joseph M. Bové
Publication year - 1992
Publication title -
international journal of systematic bacteriology
Language(s) - English
Resource type - Journals
eISSN - 1070-6259
pISSN - 0020-7713
DOI - 10.1099/00207713-42-2-220
Subject(s) - mycoplasma hyopneumoniae , biology , phylogenetic tree , 16s ribosomal rna , mollicutes , mycoplasma , homology (biology) , ribosomal rna , microbiology and biotechnology , mycoplasmataceae , gene , nucleic acid sequence , genetics , phylogenetics , sequence analysis
The nucleotide sequence of the 16S rRNA gene of Mycoplasma flocculare was determined and was compared with the sequence of a related porcine mycoplasma, Mycoplasma hyopneumoniae. While the overall level of DNA-DNA homology was approximately 11%, sequence alignment of the two 16S rRNA genes yielded a homology value of more than 95%, emphasizing the highly conserved nature of the 16S rRNA gene. Multiple sequence alignments with other mollicutes indicated that M. flocculare, M. hyopneumoniae, and Mycoplasma hyorhinis form a subcluster within the fermentans phylogroup, and this subcluster is distinct from the Mycoplasma pneumoniae phylogroup. Thus, the three mycoplasmas isolated from porcine respiratory systems exhibit phylogenetic similarities.