Open AccessIsolation and Characterization of an Anaerobic, Cellulolytic Microorganism, Acetivibrio cellulolyticus gen. nov., sp. nov.
Author(s) -
G. B. Patel,
Ayesha Khan,
Brian Agnew,
J. Ross Colvin
Publication year - 1980
Publication title -
international journal of systematic bacteriology
Language(s) - English
Resource type - Journals
eISSN - 1070-6259
pISSN - 0020-7713
DOI - 10.1099/00207713-30-1-179
Subject(s) - cellobiose , bacteria , cellulose , fermentation , microbiology and biotechnology , biology , salicin , agar , agar plate , acetic acid , cellulase , food science , botany , biochemistry , genetics
A new genus of cellulolytic, gram-negative, nonsporeforming, anaerobic bacteria is described. The colonies produced by these bacteria on cellulose agar were round, clear, translucent, and cream-colored and had an undulate margin. Single cells of the bacterium were straight to slightly curved rods 0.5 to 0.8 \u3bcm wide by 4 to 10 \u3bcm long and were motile by means of a single flagellum located one-third of the distance from the end of the cell. Among the various substrates tested, only cellulose, cellobiose, and salicin were able to support growth. The major fermentation products of cellobiose or cellulose degradation were acetic acid, hydrogen, and carbon dioxide. The deoxyribonucleic acid base composition of the type species was 38 mol% guanine plus cytosine. The name Acetivibrio is proposed for this new genus, which is placed in the family Bacteroidaceae. The type species, Acetivibrio cellulolyticus sp. nov., is named on the basis of its cellulolytic activity. The type strain of A. cellulolyticus is CD2 (= NRC 2248).Peer reviewed: NoNRC publication: Ye