High-resolution analysis of cis -acting regulatory networks at the α-globin locus | Zendy

Jim R. Hughes | Zendy; Karen M. Lower | Zendy; Ian Dunham | Zendy; Stephen Taylor | Zendy; Marco De Gobbi | Zendy; Jacqueline A. Sloane-Stanley | Zendy; Simon J. McGowan | Zendy; Jiannis Ragoussis | Zendy; Douglas Vernimmen | Zendy; Richard J. Gibbons | Zendy; Douglas R. Higgs | Zendy

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High-resolution analysis of cis -acting regulatory networks at the α-globin locus

Author(s) -

Jim R. Hughes,

Karen M. Lower,

Ian Dunham,

Stephen Taylor,

Marco De Gobbi,

Jacqueline A. Sloane-Stanley,

Simon J. McGowan,

Jiannis Ragoussis,

Douglas Vernimmen,

Richard J. Gibbons,

Douglas R. Higgs

Publication year - 2013

Publication title -

philosophical transactions of the royal society b biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.753

H-Index - 272

eISSN - 1471-2970

pISSN - 0962-8436

DOI - 10.1098/rstb.2012.0361

Subject(s) - locus (genetics) , globin , biology , genetics , computational biology , evolutionary biology , gene

We have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000 kb), the 4C approach provides a comprehensive, high-resolution analysis of a specific locus with the aim of defining, in detail, the cis-regulatory elements controlling a single gene or gene cluster. Using the human α-globin locus as a model, we detected all known local and long-range interactions with this gene cluster. In addition, we identified two interactions with genes located 300 kb (NME4) and 625 kb (FAM173a) from the α-globin cluster

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