Open AccessStudies in the Biochemistry of Micro-organisms. Part VIII.—The Estimation of Kojic Acid
Author(s) -
J. H. Birkinshaw,
Harold Raistrick
Publication year - 1931
Publication title -
philosophical transactions of the royal society of london. series b, containing papers of a biological character
Language(s) - English
Resource type - Journals
eISSN - 2053-9266
pISSN - 0264-3960
DOI - 10.1098/rstb.1931.0019
Subject(s) - chemistry , iodine , gluconic acid , chromatography , sodium , biochemistry , organic chemistry
In the estimation of the residual glucose in the metabolic products ofAspergillus parasiticus Speare, it was found that very much lower results were given by the copper reduction method of Shaffer and Hartmann (1921) than by the iodine absorption method of Hinton and Macara (1924). In the latter method, which in all essential details is the same as that previously described by Willstätter and Schudel (1918), glucose is oxidized by iodine in alkaline solution, the only product arising from the glucose being gluconic acid (as the sodium salt) according to the equation: CH2 OH . (CHOH)4 . CHO + I2 + 3NaOH = CH2 OH . (CHOH)4 COONa + 2NaI + 2H2 O.