Critical factors determining the quantification capability of matrix-assisted laser desorption/ionization– time-of-flight mass spectrometry | Zendy

Chia-Chen Wang | Zendy; YinHung Lai | Zendy; Yumeng Ou | Zendy; HuanTsung Chang | Zendy; YiSheng Wang | Zendy

Open Access

Critical factors determining the quantification capability of matrix-assisted laser desorption/ionization– time-of-flight mass spectrometry

Author(s) -

Chia-Chen Wang,

YinHung Lai,

Yumeng Ou,

HuanTsung Chang,

YiSheng Wang

Publication year - 2016

Publication title -

philosophical transactions of the royal society a mathematical physical and engineering sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.074

H-Index - 169

eISSN - 1471-2962

pISSN - 1364-503X

DOI - 10.1098/rsta.2015.0371

Subject(s) - mass spectrometry , ionization , time of flight mass spectrometry , matrix (chemical analysis) , materials science , analytical chemistry (journal) , physics , chemistry , chromatography , ion , quantum mechanics

Quantitative analysis with mass spectrometry (MS) is important but challenging. Matrix-assisted laser desorption/ionization (MALDI) coupled with time-of-flight (TOF) MS offers superior sensitivity, resolution and speed, but such techniques have numerous disadvantages that hinder quantitative analyses. This review summarizes essential obstacles to analyte quantification with MALDI-TOF MS, including the complex ionization mechanism of MALDI, sensitive characteristics of the applied electric fields and the mass-dependent detection efficiency of ion detectors. General quantitative ionization and desorption interpretations of ion production are described. Important instrument parameters and available methods of MALDI-TOF MS used for quantitative analysis are also reviewed.This article is part of the themed issue 'Quantitative mass spectrometry'.

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