Open AccessThe oxidation-reduction potentials of cytochrome C
Author(s) -
David Ezra Green
Publication year - 1934
Publication title -
proceedings of the royal society of london. series b, containing papers of a biological character
Language(s) - English
Resource type - Journals
eISSN - 2053-9185
pISSN - 0950-1193
DOI - 10.1098/rspb.1934.0015
Subject(s) - cytochrome , cytochrome c , coenzyme q – cytochrome c reductase , yeast , cytochrome c1 , chemistry , cytochrome c peroxidase , reduction potential , biochemistry , inorganic chemistry , enzyme , mitochondrion
Coolidge (1932) studied the oxidation-reduction potentials of cytochrome C. His chief results may be summarized as follows. (1) The Eo of cytochrome either in yeast cells or in artificial preparations is the same, viz., +0·260 volts. (2) In solutions of cytochrome C prepared by extraction of yeast with ammonia and by Keilin's method (1930), hæmatin iron accounts for only one-third of the total iron; the rest of the iron is in tire form of a loose iron-protein complex. (3) The low concentration of cytochrome in the living cell and the positive potentials which its solutions record make it unlikely that cytochrome can function as a respiratory catalyst. The results of Coolidge are open to serious criticism.