Open AccessCrystalline chlorocruorin
Author(s) -
Jean Roche,
H. Munro Fox
Publication year - 1933
Publication title -
proceedings of the royal society of london. series b, containing papers of a biological character
Language(s) - English
Resource type - Journals
eISSN - 2053-9185
pISSN - 0950-1193
DOI - 10.1098/rspb.1933.0078
Subject(s) - pipette , crystallization , capillary action , crown (dentistry) , chromatography , chemistry , anatomy , materials science , biology , composite material , organic chemistry
Chlorocruorin is a respiratory pigment, closely allied to hæmoglobin, which occurs only in the blood of certain polychæte worms (Fox, 1926, 1932, 1933). We have now succeeded in crystallizing chlorocruorin. A description is given below of the method of crystallization, of the nature of the crystals, of the absorption spectrum of pure oxychlorocruorin, and of its elementary analysis. Blood was extracted fromSpirographis spallanzanii at Tamaris. Pure blood was obtained by drying the animal, especially the crown, with filter paper, amputating the crown as near as possible to the body, rejecting the body, and then sucking the blood out of the the cut vessels in the base of the crown into a capillary pipette with a rudder teat. In tins way more than 1/20 c.c. of undiluted blood could be obtained from each large individual.