Open AccessThe effect of spaced radiation on tissue cultures in vitro
Author(s) -
F. G. Spear
Publication year - 1932
Publication title -
proceedings of the royal society of london. series b, containing papers of a biological character
Language(s) - English
Resource type - Journals
eISSN - 2053-9185
pISSN - 0950-1193
DOI - 10.1098/rspb.1932.0022
Subject(s) - mitosis , radium , radiation , tissue culture , in vitro , biology , radiochemistry , nuclear medicine , andrology , chemistry , optics , physics , microbiology and biotechnology , medicine , genetics
In a paper by Canti Spear (1929) it was shown that exposure of tissue cultures to 100 mg. Radium* (element)for 2½ minutes at 0·5 cm. (platinum filter 0·5mm.) produced a fall in mitosis followed by a compensatory increase, after which mitosis became normal, fig. 1. The same paper described the variation in mitotic activity at different periods after exposure to the same radium for 9 minutes. From the data given it is possible by interpolation, to construct a “mitosis curve” for the 6 hours following an exposure of 5 minutes’ duration, fig. 2. The following experiments were made in order to study the effect of two irradiations of 2½ minutes each, separated by two different time periods.