Open AccessThe vital staining of normal and malignant cells.-II. The staining of malignant tumours with trypan blue
Author(s) -
R. J. Ludford
Publication year - 1929
Publication title -
proceedings of the royal society of london. series b, containing papers of a biological character
Language(s) - English
Resource type - Journals
eISSN - 2053-9185
pISSN - 0950-1193
DOI - 10.1098/rspb.1929.0021
Subject(s) - trypan blue , staining , stain , vital stain , pathology , malignant cells , positive staining , blue light , chemistry , medicine , cancer , biochemistry , cell , materials science , optoelectronics
In the attempt to discover a rational chemotherapeutic treatment tor cancer, the influence of various dyes on malignant growths has been investigated. There has resulted a considerable difference of opinion as to the effect ofintra-vitam staining upon tumour growth. Since many acid dyes when injected into the living animal readily stain certain types of cells, the present investigation was carried out primarily to determine whether malignant cells stain intra-vitally with trypan blue, as do their normal prototypes. i.Previous Work .—The dyes which have been employed most in vital staining are tam in blue and trypan blue. According to von Möllendorff (1921) isamin blue is precipitated at the site of injection, and produced a localised vital coloration; it slowly passes into solution again and results in time in a general staining of the animal. Trypan blue, on the other hand, is one of the most suitable of the acid dyes for effecting direct intra-vitam staining.