Open AccessHæmolysis by brilliant-green and serum
Author(s) -
Eric Ponder
Publication year - 1928
Publication title -
proceedings of the royal society of london. series b, containing papers of a biological character
Language(s) - English
Resource type - Journals
eISSN - 2053-9185
pISSN - 0950-1193
DOI - 10.1098/rspb.1928.0058
Subject(s) - lysin , chemistry , bovine serum albumin , albumin , serum albumin , globulin , sodium , biochemistry , chromatography , endocrinology , biology , organic chemistry , escherichia coli , bacteriophage , gene
In earlier papers of this series (1, 2) I have described the rapid hæmolysis which results when small quantities of normal serum are added to systems containing sodium taurocholate or sodium glycocholate as lysins. This obscure phenomenon depends entirely on the order in which the cells, the lysin and the serum are mixed. If the lysin is added to the cells first and the serum added afterwards, the acceleration of hæmolysis occurs in suitable cases, whereas if the serum is mixed with the cells first and the lysin added afterwards, no acceleration,but an inhibition of hæmolysis, is the result. The effect of the addition of serum is similar for all types of mammalian red cells. The component of the serum which is responsible for the acceleration appears to be a protein, for either serum albumin, serum globulin, or hæmoglobin can bring about the effect, although egg albumin and gelatin are in effective. There is also some evidence that the acceleration on the addition of serum occurs with the lysins sodium oleate and potassium oleate, as well as with the bile salts. (3). In 1914 Browning and Mackie (4) described an action which occurs in hæmolytic systems containing red cells, serum, and brilliant-green (tetra-ethyl-diamino-triphenyl- methane sulphate). The addition of small quantities of serum to systems containing red cells sensitised with the dye results in a very rapid hæmolysis; if, however, the serum is mixed either with the dye itself before the cells are added, or with the cells before the dye is added, inhibition of the slow hæmolysis which is produced by the brilliant-green occurs. The acceleration is therefore dependent on the order in which the components of the system are mixed. A further investigation of the problem will be found in a paper by Mackie (5), in which it is shown that the serum component responsible for the acceleration is a protein, and that each of the protein fractions produces an effect. Egg albumin and gelatin do not bring about the acceleration. Dobner's violet, malachite green, ethyl violet, and methyl violet can be subsituted for the brilliant-green.