Open AccessThe measurement of percentage hæmolysis.—ΙΙ
Author(s) -
Eric Ponder
Publication year - 1927
Publication title -
proceedings of the royal society of london. series b, containing papers of a biological character
Language(s) - English
Resource type - Journals
eISSN - 2053-9185
pISSN - 0950-1193
DOI - 10.1098/rspb.1927.0013
Subject(s) - lysin , simple (philosophy) , suspension (topology) , dilution , population , chemistry , mathematics , expression (computer science) , principal (computer security) , chromatography , statistics , thermodynamics , pure mathematics , physics , biochemistry , computer science , demography , philosophy , epistemology , escherichia coli , bacteriophage , homotopy , sociology , gene , programming language , operating system
In previous papers (1, 2, 3) we have shown that the reaction between a simple hæmolysin, such as saponin or one of the bile salts or soaps, can be described by an expression not unlike that for a monomolecular reaction, provided that it is recognised that this reaction takes place among a population of cells each member of which possesses a different resistance to the lysin, the resistances being distributed according to a frequency curve. In the papers quoted it has been shown that such a conception of the reaction accounts in every way for the time-dilution curves for the simple lysins, and for the phenomena observed when the concentration of cell suspension is varied ; the percentage hæmolysis curves alone remain to be discussed in full, although in (1) it has been demonstrated that the principal properties of these curves may be explained on the above assumptions.