Open AccessProbing the chemical constituents of Cassia javanica and its in vitro analyses as a potent drug
Author(s) -
Muhammad Abdul Haleem,
Qurrat ul-Ain,
Muhammad Saadiq,
Muhammad Iqbal,
Hussain Gulab,
Sajid Ali,
Sabir Khan
Publication year - 2022
Publication title -
royal society open science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.84
H-Index - 51
ISSN - 2054-5703
DOI - 10.1098/rsos.211626
Subject(s) - proteus mirabilis , klebsiella pneumoniae , phytochemical , glycoside , chemistry , minimum inhibitory concentration , ethyl acetate , cassia , levofloxacin , traditional medicine , crude drug , proteus , antimicrobial , chromatography , stereochemistry , biochemistry , organic chemistry , antibiotics , escherichia coli , medicine , traditional chinese medicine , pathology , gene , alternative medicine
The phytochemical screening of the crude methanolic stem extracts ofCassia javanica plant showed the presence of different classes of organic compounds like alkaloids, tannins, flavonoids, saponins, phlobatanins, steroids, anthraquinone and cardiac glycoside. The starching frequencies of these functional groups were determined from FT-IR spectroscopic data. The crude and their fractions were examined for antibacterial potential againstKlebsiella pneumoniae andProteus mirabilis . The antibacterial assay showed maximum zone of inhibition for ethyl acetate fraction, i.e. 20 mm againstProteus mirabilis and 18 mm againstKlebsiella pneumoniae in the comparison with Levofloxacin used as standard (40 mm). Meanwhile for methanolic crude extract, the inhibition zone was recorded 14 mm againstKlebsiella pneumoniae and 22 mm againstProteus mirabilis . The minimum inhibitory concentrations and minimum bactericidal concentrations were recorded as 187.5 µg ml−1 againstProteus mirabilis and 93.75 µg ml−1 againstKlebsiella pneumoniae. The scavenging free radical assay was noted as 69.61% at the concentration of 100 ppm.