Open AccessAn electronic circular dichroism spectroscopy method for the quantification of L- and D-amino acids in enantiomeric mixtures
Author(s) -
Ruiwen Ding,
Jianxi Ying,
Yufen Zhao
Publication year - 2021
Publication title -
royal society open science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.84
H-Index - 51
ISSN - 2054-5703
DOI - 10.1098/rsos.201963
Subject(s) - circular dichroism , enantiomer , chemistry , chromatography , absolute configuration , enantiomeric excess , chirality (physics) , amino acid , optical rotation , molecule , calibration curve , quantitative analysis (chemistry) , amino acid analysis , analytical chemistry (journal) , stereochemistry , organic chemistry , chiral symmetry , physics , enantioselective synthesis , biochemistry , detection limit , nambu–jona lasinio model , quantum mechanics , quark , catalysis
Rapid quantitative analysis of single chiral amino acid (aa) was achieved using circular dichroism (CD) with data analysis by standard calibration curve. The absolute concentrations of D- and L-aas in enantiomeric mixtures were determined by CD and achiral liquid chromatography (LC) method. It is worth noting that CD and LC were used independently, not online LC/CD in this study. The errors of the experimental results were less than 10%. The method is also applicable to the quantification of non-aa chiral molecules, such as chiral nucleoside and chiral quinine. With this study, we provide a new method for the chiral quantitative analysis of enantiomeric aas mixtures.