tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging | Zendy

Daniel J. Nieves | Zendy; Geva Hilzenrat | Zendy; Jason Tran | Zendy; Zhengmin Yang | Zendy; Hugh H. MacRae | Zendy; Matthew A. B. Baker | Zendy; J. Justin Gooding | Zendy; Katharina Gaus | Zendy

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tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging

Author(s) -

Daniel J. Nieves,

Geva Hilzenrat,

Jason Tran,

Zhengmin Yang,

Hugh H. MacRae,

Matthew A. B. Baker,

J. Justin Gooding,

Katharina Gaus

Publication year - 2019

Publication title -

royal society open science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.84

H-Index - 51

ISSN - 2054-5703

DOI - 10.1098/rsos.191268

Subject(s) - labelling , dna , covalent bond , protein tag , dna origami , computational biology , immunological synapse , chemistry , biophysics , biology , microbiology and biotechnology , biochemistry , immune system , gene , genetics , fusion protein , recombinant dna , t cell receptor , organic chemistry , t cell

Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle.

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