Open AccessNanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy
Author(s) -
Graeme E. Johnstone,
Gemma S. Cairns,
Brian Patton
Publication year - 2019
Publication title -
royal society open science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.84
H-Index - 51
ISSN - 2054-5703
DOI - 10.1098/rsos.190589
Subject(s) - optics , adaptive optics , resolution (logic) , point spread function , nanodiamond , image resolution , microscopy , microscope , photon , diamond , two photon excitation microscopy , materials science , temporal resolution , physics , optoelectronics , computer science , fluorescence , artificial intelligence , composite material
Particles of diamond in the 5–100 nm size range, known as nanodiamond (ND), have shown promise as robust fluorophores for optical imaging. We demonstrate here that, due to their photostability, they are not only suitable for two-photon imaging, but also allow significant resolution enhancement when combined with computational super-resolution techniques. We observe a resolution of 42.5 nm when processing two-photon images with the Super-Resolution Radial Fluctuations algorithm. We show manipulation of the point-spread function of the microscope using adaptive optics. This demonstrates how the photostability of ND can also be of use when characterizing adaptive optics technologies or testing the resilience of super-resolution or aberration correction algorithms.