Open AccessBacterial bioluminescence onset and quenching: a dynamical model for aquorum sensing-mediated property
Author(s) -
D. Delle Side,
V. Nassisi,
C. Pennetta,
Pietro Alifano,
Marco Di Salvo,
Adelfia Talà,
Aleksei V. Chechkin,
Flavio Seno,
Antonio Trovato
Publication year - 2017
Publication title -
royal society open science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.84
H-Index - 51
ISSN - 2054-5703
DOI - 10.1098/rsos.171586
Subject(s) - bioluminescence , vibrio harveyi , quorum sensing , autoinducer , biological system , vibrio , quorum quenching , light emission , bacteria , bacterial growth , biophysics , quenching (fluorescence) , chemistry , biology , physics , microbiology and biotechnology , biofilm , chemical physics , biochemistry , optoelectronics , fluorescence , optics , genetics
We present an effective dynamical model for the onset of bacterial bioluminescence, one of the most studied quorum sensing-mediated traits. Our model is built upon simple equations that describe the growth of the bacterial colony, the production and accumulation of autoinducer signal molecules, their sensing within bacterial cells, and the ensuing quorum activation mechanism that triggers bioluminescent emission. The model is directly tested to quantitatively reproduce the experimental distributions of photon emission times, previously measured for bacterial colonies of Vibrio jasicida , a luminescent bacterium belonging to the Harveyi clade, growing in a highly drying environment. A distinctive and novel feature of the proposed model is bioluminescence ‘quenching’ after a given time elapsed from activation. Using an advanced fitting procedure based on the simulated annealing algorithm, we are able to infer from the experimental observations the biochemical parameters used in the model. Such parameters are in good agreement with the literature data. As a further result, we find that, at least in our experimental conditions, light emission in bioluminescent bacteria appears to originate from a subtle balance between colony growth and quorum activation due to autoinducers diffusion, with the two phenomena occurring on the same time scale. This finding is consistent with a negative feedback mechanism previously reported for Vibrio harveyi .