Open AccessBifunctional fluorescent probes for detection of amyloid aggregates and reactive oxygen species
Author(s) -
Lisa-Maria Needham,
Judith Weber,
James W. B. Fyfe,
Omaru M. Kabia,
Do Dd,
Ewa Klimont,
Yu Zhang,
Margarida Rodrigues,
Christopher M. Dobson,
Sonia Ghandi,
Sarah E. Bohndiek,
Thomas N. Snaddon,
Steven F. Lee
Publication year - 2018
Publication title -
royal society open science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.84
H-Index - 51
ISSN - 2054-5703
DOI - 10.1098/rsos.171399
Subject(s) - bifunctional , fluorescence , benzothiazole , amyloid (mycology) , reactive oxygen species , chemistry , biophysics , oxidative stress , protein aggregation , amyloid β , biochemistry , biology , disease , medicine , pathology , physics , quantum mechanics , catalysis , inorganic chemistry
Protein aggregation into amyloid deposits and oxidative stress are key features of many neurodegenerative disorders including Parkinson's and Alzheimer's disease. We report here the creation of four highly sensitive bifunctional fluorescent probes, capable of H 2 O 2 and/or amyloid aggregate detection. These bifunctional sensors use a benzothiazole core for amyloid localization and boronic ester oxidation to specifically detect H 2 O 2 . We characterized the optical properties of these probes using both bulk fluorescence measurements and single-aggregate fluorescence imaging, and quantify changes in their fluorescence properties upon addition of amyloid aggregates of α-synuclein and pathophysiological H 2 O 2 concentrations. Our results indicate these new probes will be useful to detect and monitor neurodegenerative disease.