Open AccessCultured cells and wing disc size of silkworm can be controlled by the Hippo pathway
Author(s) -
Zi Liang,
Yingjie Lu,
Ying Qian,
Liyuan Zhu,
Sulan Kuang,
Fei Chen,
Yongjie Feng,
Xiaolong Hu,
Guangwen Cao,
Renyu Xue,
Chengliang Gong
Publication year - 2018
Publication title -
open biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.078
H-Index - 53
ISSN - 2046-2441
DOI - 10.1098/rsob.180029
Subject(s) - biology , hippo signaling pathway , microbiology and biotechnology , cytokinesis , cell growth , cell division , ubiquitin ligase , cell cycle , gene , signal transduction , cell , genetics , ubiquitin
Hippo signalling represents a cell proliferation and organ-size control pathway. Yorki (Yki), a component of the Hippo pathway, induces the transcription of a number of targets that promote cell proliferation and survival. The functions of Yki have been characterized in Drosophila and mammals, while there are few reports on silkworm, Bombyx mori In the present study, we found that BmYki3 facilitates cell migration and cell division, and enlarges the cultured cell and wing disc size. Co-immunoprecipitation results indicated that BmYki3 may interact with thymosin, E3 ubiquitin-protein ligase, protein kinase ASK1, dedicator of cytokinesis protein 1, calcium-independent phospholipase A2 and beta-spectrin. RNA-seq results indicated that 4444 genes were upregulated and 10 291 genes were downregulated after BmYki3 was overexpressed in the cultured cells. GO annotation indicated that the up/downregulated genes were enriched in 268/382 GO terms ( p < 0.01); KEGG analysis showed that the up/downregulated genes were enriched in 49/101 pathways. These findings provided novel information to understand the functions of BmYki3 in a cell proliferation and organ-size control pathway.